Clinical Chemistry · 1968 · 27 citations · 0 references
Natural SciencesBiochemistryAnalytical ChemistryChromatographic AnalysisMass Spectrometric AnalysisAmino Acid ChemistryAnalytical ChromatographyChromatographyAmino Acid AnalysisAmino Acid ChromatographyAmino Acid Separation TechniquesAmino AcidsOptimum TemperatureSensitivityTemperature Optimum
Abstract The reaction of 2,4,6-trinitrobenzenesulfonic acid (TNBS) with amino acids was investigated by a static system to develop optimum conditions for application to automated amino acid chromatography. TNBS was found to be a very simple and sensitive reagent, easier to use than ninhydrin. The reaction occurs quite rapidly at room temperature, eliminating the requirement of a heating bath. The major disadvantage is a much lower sensitivity to the imino acid proline than to the amino acids.