Conditions leading to flower formation on excised Begonia fragments cultured in vitro

Abstract

Fragments of leaves, petioles and floral stalks of several species and varieties of Begonia have been cultured in vitro on denned media. The presence of a cytokinin, adenine and an auxin was found necessary for optimum bud formation, especially on petioles and floral stalks. Flower buds could be produced on leaves and floral stalks, but not on petioles. They were of the male type.