Substance P-immunoreactive nerves in the human cornea and iris.

Abstract

Human corneas and irides obtained from corneal surgery or sector iridectomies were processed according to the indirect immunofluorescence technique for the demonstration of substance P (SP) immunofluorescence by means of a monoclonal SP antibody. SP immunofluorescence was observed in varicose fibers of the corneal epithelium and nerve trunks in the corneal stroma. SP-immunoreactive nerve fibers in the iris were detected mainly in the pupillary margin in both dilator and sphincter areas.