Purification of Rat Heart and Rat Liver Citrate Synthases

Abstract

Abstract The citrate synthases from rat heart and rat liver have been purified. These homogeneous proteins have a molecular weight of about 1 x 105 and are composed of 2 apparently identical subunits. Kinetic constants for acetyl coenzyme A and oxaloacetate and the Ki for ATP are the same for both enzymes. Immunological studies using antibody to the rat heart enzyme also indicate that the enzymes are identical.