Simultaneous detection of <i>Tomato spotted wilt virus</i>,<i>Dahlia mosaic virus</i> and <i>Chrysanthemum stunt viroid</i> by multiplex RT-PCR in dahlias and their distribution in Japanese dahlias

Abstract

The multiplex RT-PCR assay described in this study is the first report of simultaneous detection of virus and viroid in dahlia. This method represents a rapid, sensitive and cost effective approach to diagnose viral infections in dahlias. A field survey validated our results, indicating that TSWV was the dominant virus found in the Kansai region, DMV in the Tohoku and Kyushu regions and CSVd in the Hokkaido region.