Multiplex PCR for Enterotoxigenic, Attaching and Effacing, and Shiga Toxin-Producing<i>Escherichia coli</i>Strains from Calves

Abstract

A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.