Genetics · 1996 · 658 citations · 13 references
Female germline chimeras are essential for dissecting tissue‑specific recessive female sterile mutations and maternal effects of recessive zygotic lethal mutations, and the FLP‑DFS technique was previously developed to generate such clones efficiently. This study aims to extend the FLP‑DFS approach to autosomes by constructing chromosomes that enable germline mosaic production outside the X chromosome. The method employs the ovo D1 female‑sterile selection together with FLP‑FRT recombination to induce site‑specific chromosomal exchange, allowing efficient generation of germline mosaics on the X chromosome and, with the new chromosomes, on autosomes. We successfully built autosomal chromosomes that permit the FLP‑DFS technique to produce germline mosaics on autosomes.
The production of female germline chimeras is invaluable for analyzing the tissue specificity of recessive female sterile mutations as well as detecting the maternal effect of recessive zygotic lethal mutations. Previously, we developed the “FLP-DFS” technique to efficiently generate germline clones. This technique uses the X-linked germline-dependent dominant female sterile mutation ovo D1 as a selection for the detection of germline recombination events, and the FLP-FRT recombination system to promote site-specific chromosomal exchange. This method allows the efficient production of germline mosaics only on the X chromosome. In this paper we have built chromosomes that allow the use of this technique to the autosomes. We describe the various steps involved in the development of this technique as well as the properties of the chromosomes utilized.
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A stable genomic source of P element transposase in Drosophila melanogaster.
Hugh M. Robertson, Christine R Preston, R W Phillis et al. · Genetics · 1988 · 1.3K citations