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The Effect of EGTA, Calcium Channel Blockers (Lanthanum Chloride and Nifedipine) and their Interaction with Abscisic Acid on Seed Germination of Brassica juncea cv. RLM-198
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1992
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BiologyAffected GerminationLanthanum ChlorideEngineeringGermination InhibitionBotanyPlant Defence ActivatorNatural SciencesCrop ProtectionAgricultural EconomicsAbscisic AcidSeed GerminationPlant PathologyCrop PhysiologyPlant Growth RegulatorPhytotoxicityPlant HormonePlant Physiology
Recent investigations have shown that abscisic acid (ABA) depends upon the availability of Ca2+ for its action in certain systems. In order to check whether ABA requires Ca2+ to inhibit seed germination, the effects of ABA, CaCl2, EGTA (a Ca2+ chelator) and lanthanum chloride and nifedipine (Ca2+ channel blockers), individually and in combination, on seed germination of Brassica juncea L. cv. RLM-198 were studied. ABA inhibited germination in a concentration dependent manner. ABA-induced inhibition was abolished with the lapse of time after application. Calcium neither affected germination nor altered the ABA-induced inhibition. EGTA. La3+ and nifedipine suppressed seed germination when tested individually and further elevated the ABA effect, in a synergistic way, when applied simultaneously. They also prevented the time-dependent reduction in ABA-induced inhibition. The findings indicate that Ca2+ is not essential for ABA to cause germination inhibition.