Gene mapping with recombinant inbreds in maize.

TLDR

Recombinant inbred maize lines enable rapid mapping of molecular probes to chromosomal positions. Two recombinant inbred families derived from F2 crosses (T232×CM37 and CO159×Tx303) allow gene mapping by determining a gene’s allelic distribution in the lines and computationally comparing it to existing loci. A near‑complete maize genetic map using isozymes and RFLPs has been generated, and the recombinant inbreds with their database provide an efficient tool for mapping new markers.

Abstract

Recombinant inbred lines of maize have been developed for the rapid mapping of molecular probes to chromosomal location. Two recombinant inbred families have been constructed from F2 populations of T232 X CM37 and CO159 X Tx303. A genetic map based largely on isozymes and restriction fragment length polymorphisms has been produced that covers virtually the entire maize genome. In order to map a new gene, an investigator has only to determine its allelic distribution among the recombinant inbred lines and then compare it by computer with the distributions of all previously mapped loci. The availability of the recombinant inbreds and the associated data base constitute an efficient means of mapping new molecular markers in maize.