Relative affinity of hemoglobin S and hemoglobin A for carbon monoxide and oxygen.

Abstract

The relative affinity constants of hemolysates from individuals with hemoglobins A, S, or AS have been measured at 37 and 26 ° C. Observed values of all hemoglobin types were the same at both temperatures, K 37 = 230, K 26 = 296. Control measurements on whole blood containing Hb A gave values of K 37 = 222. The small but significant difference between K 37 values measured in whole blood and in hemolysates may be a result of the greater increase of MetHb in the hemolysates during the in vitro equilibration.