<b>SMALL DERMATAN SULFATE PROTEOGLYCANS IN HUMAN EPIDERMIS AND </b><b>DERMIS </b>

Abstract

Proteoglycans were isolated from human epidermis and dermis by extraction with 4 M guanidinium chloride, then fractionated on a DEAE-cellulose column. Pure dermatan sulfate proteoglycan (DSPG) was achieved by differential ethanol precipitation. The M, values of the epidermal and dermal DSPGs were 130 and 66 K, respectively. Alkaline borohydride treatment of DSPGs released glycosaminoglycan