Journal of Clinical Microbiology · 1994 · 32 citations · 13 references
Veterinary MedicineMicrobiologyVeterinary MicrobiologyDiagnostic MicrobiologyMolecular VirologyVirologyInfectious Disease DetectionInfectious Disease Molecular TestingPathogen DetectionVeterinary Diagnostic MicrobiologyVeterinary VirologyViral DiagnosticsEpidemiological Tracing Of PathogensPcr-based DetectionPolymerase Chain Reaction (Pcr) AssaysPolymerase Chain Reaction (Pcr) DiagnosticsSwine Viral DiagnosticsTissue Culture MediaViral Disease DiagnosticsVirus Detection Assays
A reverse transcriptase-PCR strategy was developed for the detection of hog cholera virus. Hog cholera virus template was amplified from tissue culture fluids and from tissues and blood of infected pigs, but not from samples containing other pestiviruses. Restriction endonuclease analysis identified samples as historic or recent isolates.
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Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Randall K. Saiki, David H. Gelfand, Susanne Stoffel et al. · Science · 1988 · 17.2K citations
Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus
Marc S. Collett, Ruby J. Larson, Conrad Gold et al. · Virology · 1988 · 483 citations