Journal of Bacteriology · 1976 · 96 citations · 31 references
Life SciencesGeneticsMolecular GeneticsEnzymologyMicrobial GeneticsStructural BiologyAntibiotic ResistanceAntibiotic Resistance GeneticsBacterial GeneticsGene MappingPhage GeneticsResistance GenesAntibiotic Resistance GenesAntibiotic Resistance MarkersBacteriophage BiologyEscherichia Coli ResistanceGenetic Map ConstructionPhage Structural Gene OrganizationResistance Gene MarkersResistance Plasmid Transfer
The genes xthA, pncA, and pabB were ordered relative to others by two- and three-factor transductional crosses with bacteriophage P1. The genes studied span 2 min (2%) of the genetic map of Escherichia coli K-12 in the clockwise sequence pheS-pfkB-xthA-pncA-gap-pabB-fadD. Eleven independently derived xth mutations were examined; all were known to affect exonuclease III and its associated endonuclease II activity, and all were mapped in the xthA region. pncA mutations were found to confer resistance to 6-aminonicotinamide, whereas some pheS mutations are known to specify resistance to p-fluorphenylalanine. xth mutations were readily transferred into other strains by selecting for these co-transducible drug resistance markers.
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Recalibrated linkage map of Escherichia coli K-12
B Bachmann, K. Brooks Low, Austin L. Taylor · Bacteriological Reviews · 1976 · 1K citations · Full text
Life Sciences, Biology, Genetics +9
Isolation of an E. coli Strain with a Mutation affecting DNA Polymerase
PAULA DE LUCIA, John Cairns · Nature · 1969 · 753 citations