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Effects of Calmodulin on Ca<sup>2+</sup>-Dependent Cl<sup>−</sup>-Sensitive Anion Channels in the <italic>Chara</italic> Plasmalemma: a Patch-Clamp Study
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1993
Year
-Sensitive Anion ChannelsAllergyHyperpolarization (Biology)MedicineSensitive Anion ChannelPhysiologyIon ChannelsElectrolyte DisturbanceMembrane BiologyElectrophysiologyInside-out Patch-clamp TechniquePatch-clamp StudyPharmacologyChara Plasmalemma-Dependent Cl
The effects of calmodulin (from spinach) on the Ca2+-dependent Cl--sensitive anion channel in the Chara plasmalemma (Okihara et al. 1991) were studied by the inside-out patch-clamp technique. The current of Cl- ions, which flowed through the channel at 1.0 μM Ca2+, tended to decrease irregularly with time. This tendency toward a decrease in the current was no longer apparent after application of calmodulin for some time. The activity of the channel was restored to a small extent or tended to increase during the early stages of application of calmodulin. Such a transient action of calmodulin on the channel activity was evident, at voltages more negative than –100 mV.