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A Microtiter Solid-Phase Radioimmunoassay for Hepatitis a Antigen and Antibody
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1976
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ImmunologyHepatitis BBiomedical EngineeringMicrotiter Solid-phase RadioimmunoassayImmunotherapyLiver BiopsyViral HepatitisHematologySerologic TestingImmunochemistryLaboratory MedicineHa AgLiver TransplantationAntibody ScreeningVaccinationHepatologyType BHepatitisImmune Electron MicroscopyMedicineHepatocellular CarcinomaAutoimmune Hepatitis
A microtiter solid‑phase radioimmunoassay for hepatitis A antigen and antibody was developed. The authors optimized reagent specificity and sensitivity, determined optimal assay conditions, and compared the assay with immune adherence hemagglutination and immune electron microscopy. The assay was more sensitive than immune adherence hemagglutination for detecting hepatitis A antigen and nearly as sensitive for antibody, correctly identifying serologic responses in type A hepatitis patients but not in type B or non‑A, non‑B hepatitis.
A microtiter solid phase radioimmunoassy for hepatitis A antigen (HA Ag) and antibody (anti-HA) was developed. The test was more sensitive than immune adherence hemagglutination for detecting HA Ag and almost as sensitive for detecting anti-HA. The specificity and sensitivity of reagents were examined and optimum conditions for the test were determined. Radioimmunoassay, immune adherence hemagglutination, and immune electron microscopy were compared for detecting anti-HA. A serologic response to HA Ag was detected in paired sera from patients with type A hepatitis but not from patients with type B or non-A, non-B hepatitis by all three techniques.