Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards.

Abstract

Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus
\nindicus. Viable spermatozoa was preserved successfully for a period of 60 days at -3S'C
\nand -196'C temperatures. Among several cryoprotectants used, a combination of DMSO (5%)
\nand glycerol (5%), and DMSO (5%) and trehalose (O.25M) showed best viability rate (75 to
\n80%) in freeze thawed spermatozoa. Activational changes in spermatozoa during acrosome
\nreaction after induction with egg water have been mentioned. The importance of cryogenic
\nstorage of spermatozoa is discussed.