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High Performance Liquid Chromatography of Unsaturated Disaccharides Produced from Chondroitin Sulfates by Chondroitinase
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1979
Year
Peak AreaEngineeringFood AnalysisGlycobiologyPolysaccharideUnsaturated Disaccharides ProducedSeparated PeaksBioanalysisAnalytical ChemistryLiquid ChromatographyChromatographyBiochemistryChromatographic AnalysisIon Pair MethodBiomolecular EngineeringChondroitin SulfatesMedicineHemicelluloseDrug Analysis
High performance liquid chromatography was performed by the ion pair method on unsaturated disaccharides produced from chondroitin sulfates by the action of chondroitinase. Completely separated peaks corresponding to deltaDi-0S, deltaDi-4S, and deltaDi-6S were obtained on a column of mu-bondapak-C18 with 0.035 M tetra-butylammonium phosphate (pH 7.54) as a mobile phase. There was a linear relationship between the ratio of the peak area and the amount of each standard tested.