Evaluation of the Antibacterial efficacy of Omeprazole with Sodium Hypochlorite as an Endodontic Irrigating Solution- An Invivo Study.

Abstract

BACKGROUND: Proton pump inhibitors are used in endodontic disinfection of root canals for elimination of enterococcus faecalis. This invivo study on Wistner Rats is carried out to determine antimicrobial efficacy of proton pump inhibitor in combination with sodium hypochlorite & Mixture of Isomer of tetracycline, acid and detergent (MTAD) against E. Faecalis Materials & Methods: Periapical lesions were induced on the 30 Incisor teeth of 30 male Wistner rats (10per group). After 28 days, root canals of each tooth were instrumented to 35# k file, during the process of instrumentation canals were irrigated with their respective irrigation solutions. Group-1: 2% CHX + 5.2% NaOCL, Group-2: MTAD(Dentsply Tulsa Dental, Tulsa, OK) + 5.2% NaOCL, Group-3: 8.5% Omeprazole (Dr Reddy's labs private limited - Hyderabad) + 5.2% NaOCL. Microbiological samples were collected by using #35 sterilized paper points after 28 days of inducing periapical lesions, Sample (S1) was collected before instrumentation and Sample (S2) was collected after instrumentation and Irrigation data were subjected to analysis of variance, followed by Newman Keuls Post Hoc test. RESULTS: Microbiological Analysis revealed significant decrease of colony forming units from S1 to S2 Samples in all the 3 groups. CONCLUSION: Our data showed that association of Omeprazole with NaOCL showed a superior antibacterial efficacy against Enterococcus faecalis in comparision with other irrigants. Key words: Sodium Hypochlorite, Chlorhexidine, Gluconate, MTAD, Omeprazole.