Comparative Analysis of Routine Histology, Immunohistochemistry, Reverse Transcriptase Polymerase Chain Reaction, and Fluorescence In Situ Hybridization in Diagnosis of Ewing Family of Tumors

Abstract

The excellent correlation of routine histologic findings in Ewing family of tumors with results on immunohistochemistry and fluorescence in situ hybridization on archival material and reverse transcriptase polymerase chain reaction on fresh FNAC specimens underscores that the traditional observation on routine histologic examination is a time-tested tool. The diagnosis of Ewing family of tumors can be validated on archival material or fresh biopsy samples, including those obtained by FNAC.