Epigenetically downregulated Semaphorin 3E contributes to gastric cancer

Abstract

// Hui Chen 1 , Guo-Hua Xie 1 , Wei-Wei Wang 1 , Xiang-Liang Yuan 1 , Wen-Ming Xing 2 , Hong-Jing Liu 1 , Jin Chen 1 , Min Dou 1 , Li-Song Shen 1 1 Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China 2 Department of Academy, Shanghai Association for Science & Technology, Shanghai 200020, China Correspondence to: Li-Song Shen, e-mail: lisongshen@hotmail.com Keywords: Sema3E, gastric cancer, epigenetics, proliferation, migration Received: January 23, 2015      Accepted: April 29, 2015      Published: May 12, 2015 ABSTRACT Axon guidance protein Semaphorin 3E (Sema3E) promotes tumor metastasis and suppresses tumor cell death. Here, we demonstrated that Sema3E was decreased in gastric cancer. Its levels were inversely associated with tumor progression. Levels of Sema3E were associated with low p300 and high class I histone deacetylase (class I HDAC). Ectopic expression of Sema3E inhibited proliferation and colony formation of gastric cancer cell lines in vitro and xenografts in vivo . Sema3E overexpression inhibited migration and invasion of gastric cancer cells, which was associated with induction of E-cadherin and reduction of Akt and ERK1/2 phosphorylation. We suggest that silencing of Sema3E contributes to the pathogenesis of gastric cancer.