Publication | Closed Access
A ratiometric lysosomal pH probe based on the coumarin–rhodamine FRET system
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Citations
57
References
2015
Year
EngineeringBiochemistryBiomedical DiagnosticsNatural SciencesMolecular BiologySingle-molecule DetectionCoumarin MoietyFret AcceptorAnalytical ChemistryBioimagingClinical ChemistryAnalytical UltracentrifugationFret DonorChemical ProbeMolecular ImagingCoumarin–rhodamine Fret SystemBiomolecular ScienceBiophysics
The ratiometric pH probe <bold>RC1</bold> was constructed by integrating a coumarin moiety as a FRET donor into a rhodamine moiety as a FRET acceptor. The probe with a p<italic>K</italic><sub>a</sub> of 4.98 could detect pH in the range of 4.20–6.00 and selectively stain lysosomes.
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