A novel in vitro transcription‐translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +11

Efficient<i>in vitro</i>synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

Douglas A. Melton, Paul A. Krieg, Michael R. Rebagliati et al. · Nucleic Acids Research · 1984 · 6.4K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +11

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

Jeffrey Vieira, Joachim Messing · Gene · 1982 · 6.1K citations

Life Sciences, Genetics, Molecular Biology +15

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

Günter Blobel, B Dobberstein · The Journal of Cell Biology · 1975 · 3K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +12

SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN <i>Escherichia coli</i> : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM

Don B. Clewell, Donald R. Helinski · Proceedings of the National Academy of Sciences · 1969 · 1.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +12