Application of Computer‐assisted Sperm Analysis in Selecting the Suitable Solution for Common Carp, <i>Cyprinus carpio</i> L., Sperm Motility

Abstract

Abstract The common carp, Cyprinus carpio L., sperm motility parameters were analyzed by using computer‐assisted sperm analysis system. The percentage of motile sperm ( MOT , %), progressively motile sperm ( PRG , %), curvilinear velocity ( VCL , µm/sec), average path velocity ( VAP , µm/sec), the wobbling index ( WOB , %), movement linearity ( LIN , %), beat cross frequency ( BCF , Hz), and amplitude of lateral head displacement ( ALH , µm) were determined. Five activation solutions (As) were used to activate sperm movement. As 1 solution: 68 mM NaCl , 50 mM urea, 0.5% bovine serum albumin ( BSA ), pH : 7.7, 181 mOsm /kg; As 2 buffer: 100 mM NaCl , 10 mM Tris, 0.5% BSA , pH : 9.0, 199 mOsm /kg; As 3 solution: 86 mM NaCl , 0.5% BSA , pH : 7.4, 167 mOsm /kg; As 4 buffer: 5 mM KCl , 45 mM NaCl , 30 mM Tris, 0.5%, pH : 8.0, 160 mOsm /kg; and As 5 solution: distilled water with the addition of 0.5% BSA , pH : 7.3, &lt;3 mOsm /kg. Among five tested solutions, a buffer with a pH of 9.0 and osmolality of approximately 200 mOsm /kg (As 2) was the most suitable. After its activation, a significant increase in MOT and ALH values was observed, which can be of importance to the effectiveness of egg fertilization .