Identification of Clones Induced by Chromosome Manipulation in Ayu <i>Plecoglossus altivelis</i> by DNA Fingerprinting with RI and Non-RI Labelled Probes

Abstract

DNA fingerprint (DNA-FP) was applied for confirmation and identification of clones induced by the suppression of the first cleavage in ayu, Plecoglossus altivelis. The samples used were 2 homozygous clonal lines, 3 successive homozygous clonal lines and 2 heterozygous clonal lines. We examined suitable enzyme/probe combinations and compared radioisotope (RI) and non-radioisotope (Non-RI) labelled probes to get clear DNA-FP. The clear DNA fingerprints were obtained by using YNZ 22, 33. 15 and 33.6 probes with Hae III, Msp I and Hinf I restriction enzymes. In these DNA-FPs, the bands were separated distinctly throughout the membrane in both higher and lower molecular weight regions. The DNA-FP bands obtained with the Non-RI labelled 33.15 probes were as intense and clearly separated as those obtained with the RI method. All the fragments were shared among individuals within the same clonal line, but the pattern of bands detected was significantly different between clonal lines. It was confirmed that DNA-FP is effective for the identification of clonal lines. DNA-FP pattern by Non-RI labelled probe would be suitable for wide applications in studies on inbreeding and identification of individuals and families.