Clinical Chemistry · 1988 · 20 citations · 0 references
PharmacyPharmaceutical AnalysisPharmacologyBiochemical PharmacologyChromatographic AnalysisClinical PharmacologyAnalytical ChromatographyArea Under The CurveChromatographic Analysis Of PharmaceuticalsChromatographyDrug DispositionLiquid ChromatographyBioanalytical ChromatographyCarbamazepine PharmacologyDrug Metabolite ProfilingEpoxide MetabolitesMetabolite PharmacokineticsMicrobore Liquid ChromatographyPeak Area EstimationPlasma Drug Concentration Monitoring
Simultaneous monitoring of carbamazepine and its metabolities is helpful in providing information on the induction process via the epoxide-diol route. The present method involves the liquid-chromatographic analysis of 1.0 mL of plasma for carbamazepine at concentrations of 0.025 to 4.0 mg/L and for both the 10,11-epoxide and 10,11-transdiol metabolites at concentrations of 0.01 to 1.0 mg/L. Weighted regression equations, expressing peak area ratios as a function of concentrations of carbamazepine and its epoxide and transdiol metabolites in the standards, were used to determine concentrations in plasma samples.