The sulphatase of ox liver. 7. The intracellular distribution of sulphatases A and B

Abstract

Previous papers in this series (Roy, 1953a(Roy, , b, 1954a Sulphatase C apparently occurs only in the microsomes (Roy, 1956, 1958), whereas sulphatases A and B appear in several cell fractions. It was originally suggested that they occurred predominantly in the mito- chondria and microsomes of rat liver (Roy, 1954b), but However, these investigations all suffered from the disadvantage that sulphatases A and B were determined as one unit, the two individual enzymes not being separately determined. It is obvious that such studies could give misleading results and that important differences in the localizations of the two enzymes might not be detected. Unfortunately, the separate determination of sul- phatases A and B in a mixture is a matter of some difficulty, especially when the situation is further complicated by the simultaneous presence of sulphatase C.