Isoelectric Focusing in Agarose: Phosphoglucomutase (PGM Locus 1) Typing

Abstract

The subphenotypes of phosphoglucomutase (PGM) was successfully typed by isoelectric focusing with a charge-balanced agarose. The resolution was as high as that obtained with polyacrylamide, with none of the drawbacks associated with the use of polyacrylamide. Exceptionally straight iso-pH lines were obtained, making interpretation of the results more reliable. Gel preparation was simple and rapid, and the time for analysis was reduced. A way of preserving the resultant zymogram is described.