Mammalian Brains Are Made of These: A Dataset of the Numbers and Densities of Neuronal and Nonneuronal Cells in the Brain of Glires, Primates, Scandentia, Eulipotyphlans, Afrotherians and Artiodactyls, and Their Relationship with Body Mass

TLDR

Comparative neurobiology has traditionally focused on brain volume and surface area, with limited neuronal density estimates mainly confined to the cerebral cortex. The authors present a comprehensive dataset of neuronal and nonneuronal cell counts and densities across 39 mammalian species to support broader evolutionary and comparative research. They compiled data on total brain cell numbers, subdivided into cerebral cortex, cerebellum, and the rest of the brain, for species spanning six clades. Analysis shows that in the rest of the brain neuronal cell size scales with body linear dimension, while neuron number does not follow a single scaling rule, so larger bodies have larger neurons in the rest of the brain but not necessarily more neurons.

Abstract

Comparative studies amongst extant species are one of the pillars of evolutionary neurobiology. In the 20th century, most comparative studies remained restricted to analyses of brain structure volume and surface areas, besides estimates of neuronal density largely limited to the cerebral cortex. Over the last 10 years, we have amassed data on the numbers of neurons and other cells that compose the entirety of the brain (subdivided into cerebral cortex, cerebellum, and rest of brain) of 39 mammalian species spread over 6 clades, as well as their densities. Here we provide that entire dataset in a format that is readily useful to researchers of any area of interest in the hope that it will foster the advancement of evolutionary and comparative studies well beyond the scope of neuroscience itself. We also reexamine the relationship between numbers of neurons, neuronal densities and body mass, and find that in the rest of brain, but not in the cerebral cortex or cerebellum, there is a single scaling rule that applies to average neuronal cell size, which increases with the linear dimension of the body, even though there is no single scaling rule that relates the number of neurons in the rest of brain to body mass. Thus, larger bodies do not uniformly come with more neurons - but they do fairly uniformly come with larger neurons in the rest of brain, which contains a number of structures directly connected to sources or targets in the body.