Electrochemical and Biological Studies on Reactivity of [VO(oda)(H₂O)₂], [Co(oda)(H₂O)₂]·H₂O, and [Ni(oda)(H₂O)₃]·1.5H₂O Towards Superoxide Free Radicals

Abstract

Abstract The reactivity of superoxide free radicals (O 2 · – ) generated electrochemically towards the oxydiacetate metal complexes, namely [VO(oda)(H 2 O) 2 ], [Co(oda)(H 2 O) 2 ] · H 2 O, and [Ni(oda)(H 2 O) 3 ] · 1.5H 2 O (oda = oxydiacetate) was examined by cyclic voltammetry. The measurements were carried out in DMSO solution using a platinum electrode. Based on the height of the anodic peak E a that corresponds to electrochemical oxidation O 2 · – → O 2 + e, in the absence and in the presence of the compounds in the mixture, their O 2 · – scavenge ability was assessed. The influence of the type of the complex was briefly discussed. H 2 O 2 was used to induce cellular injury in a mouse hippocampal cell line (HT22). The cytoprotection of chemical compounds was tested at the mitochondrial (MTT test) and plasma membrane level (LDH leakage). Dose‐dependent effect (10 and 100 μM of the complex) of investigated compounds was observed.