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Effect of Intracellular ATP Levels on the Light-Induced H<sup>+</sup> Efflux from Intact Cells of <italic>Cyanidium caldarium</italic><xref ref-type="fn" rid="fn1"><sup>1</sup></xref>
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1984
Year
Intact CellsPhotobiologyLight-induced H+ EffluxRedox BiologyCellular PhysiologyPhototropinOxidative StressLight-induced HIntracellular Atp LevelsPhotosynthesisBiophysicsHealth SciencesLight RegulationBiochemistryPhotochemistryPlasma Membrane AtpasePhysiologyPhotoprotectionTriton X-100Cellular BiochemistryMetabolismMedicine
The light-induced H+ efflux observed at acidic pH in Cyanidium cells was shown to be an active H+ transport depending on the intracellular ATP produced by cyclic photo-phosphorylation. Triton X-100 was found to act as an effective uncoupler in intact Cyanidium cells without collapsing the pH gradient across the plasma membrane. Triton X-100 at 0.015% significantly reduced the intracellular ATP levels, stimulated the p-BQ, Hill reaction and completely inhibited the light-induced H+ efflux. Inhibition of the H+ efflux by Triton X-100 correlated well with the depression of the apparent rale of light-induced ATP synthesis as well as the decrease in the intracellular ATP level in light. The light-induced H+ efflux was completely inhibited by diethylstilbestrol, a specific inhibitor of plasma membrane ATPase, without any changes in the intracellular ATP level, thereby suggesting the participation of the plasma membrane ATPase in the light-induced H+ efflux.