Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.

TLDR

The study characterizes the anti‑My‑10 antibody’s binding to human hematopoietic cells, showing that My‑10 is expressed specifically on immature marrow cells, including progenitors. The anti‑My‑10 monoclonal antibody was generated against the KG‑1a myeloid cell line and selected for lack of reactivity with mature granulocytes. Anti‑My‑10 binds a 115‑kDa surface protein expressed on immature marrow progenitors and a subset of leukemic cells, enabling their identification, purification, and leukemia subclassification.

Abstract

The anti-My-10 mouse monoclonal antibody was raised against the immature human myeloid cell line KG-1a and was selected for nonreactivity with mature human granulocytes. Anti-My-10 immunoprecipitated a KG-1a cell surface protein with an apparent Mr of approximately 115 kD. We describe the binding of this antibody to human hematopoietic cell types and show that My-10 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. My-10 is also expressed by leukemic marrow cells from a subpopulation of patients. Thus, this antibody allows the identification and purification of hematopoietic progenitor cells from normal human marrow and the subclassification of leukemia.