Staining of paraffin-embedded plant material in safranin and fast green without prior removal of the paraffin

Abstract

A rapid and efficient method for staining sections of paraffin-embedded plant tissues in safranin and fast green without prior removal of the paraffin is described. The quality of the resulting preparations is equal to, or better than, that obtained by conventional methods. This method results in substantial saving of time and expense. Key words: staining technique, paraffin, safranin, fast green.