PubMed · 1993 · 12 citations · 0 references
Life SciencesGeneticsMolecular BiologyDna ReplicationMolecular BiotechnologyNucleic Acid BiochemistryDna Polymerase FunctionPlasmid ReplicationPolymerase Chain ReactionReplication FidelityDna Polymerase FidelityDna Polymerase IRibonucleotide Incorporation
The fidelity of DNA replication with respect to misincorporation of ribonucleotides into DNA has been studied. Primed M13 DNA was replicated by E. coli DNA polymerase I in the presence of four dNTPs, a single rNTP and Mg2+ as a cofactor. All four rNTPs were incorporated into DNA in place of the corresponding dNTPs when rNTP/dNTP ratio was 1000:1. The frequency of rNTP misincorporation was C > G > A > U.