BIOCHEMICAL AND HEMATOLOGICAL PROFILE IN CATTLE EFFECTIVE

Abstract

ABSTRACT Introduction: Metabolic profile represents nutritional-metabolic integrity evaluation in an animal effective through biochemical and hematological tests. Aims: The purpose of this study was to evaluate biochemical and hematological changes in a cattle effective, part of the metabolic profile assessment. Materials and methods: Blood analysis was carried out inside the National Reference Laboratory in Animal Wellbeing Evaluation from the Institute of Diagnosis and Animal Health Bucharest. Blood samples were collected using EDTA vacutainers for hematologic examination and without anticoagulant for biochemical analysis. Hematological investigations were carried out using an automated analysis machine AcT5diff CP and biochemical investigations were carried out through classic methods such as spectrophotometry as well as modern methods by VETSCAN VS2 analyzer. Results: Biochemical results showed an increase in total protein with values ranging from 8.6 to 9.53 g/dl, an increased activity of  Gama-Glutamil-Transferase with values ranging from 21 to 34 UI/L and an increase in total bilirubin levels from 0.3 to 2.4 mg/dl. Hematologic examination revealed leukocytosis from 10.000 to 19.500/mm³ with lymphocytosis from 60 to 78%. Other blood parameters showed normal reference values for the species and physiological category of investigated animals. Conclusions: Blood tests allow early recognition of biochemical and hematological anomalies which contributes to continuous metabolic surveillance of a large animal effective.