The human int-1 gene is located at chromosome region 12q12-12q13 and is not rearranged in myxoid liposarcoma with t(12;16) (q13;p11).

Abstract

The mouse cellular oncogene int-1 is often transcriptionally activated as a consequence of nearby proviral insertions in mouse mammary tumors. A highly conserved sequence has been found in the human genome, called int-1 gene, the role of which in human tumors is not known. By somatic hybrids, the human int-1 gene has been assigned to the segment 12q14-12pter. Using a genomic DNA clone containing the fourth exon of the human int-1 gene we have mapped the human int-1 gene to 12q12-12q13. To determine whether this gene, which is located close to the 12q13 breakpoint associated with myxoid liposarcoma, is rearranged in these tumors, we have performed Southern blot analysis of DNA from myxoid liposarcomas carrying the translocation t(12;16) (q13;p11). In the two tumors investigated, the translocation does not disrupt the int-1 gene.