Publication | Closed Access
The combination of the overpressured layer chromatography and bioautography and its applications to the analysis of molecules influencing cell proliferation
17
Citations
0
References
2003
Year
Complex SeparationEngineeringCell CultureCell ProliferationCellular PhysiologySeparation ScienceOverpressured Layer ChromatographyBioanalysisAnalytical ChemistryLiquid ChromatographyClinical ChemistryChromatographyCell DivisionBiochemistryBioassay-guided IsolationOplc SeparationPharmacologyCell BiologyChromatographic AnalysisBiotechnologyMicrobiologyMedicine
BioArena is a complex separation and detection system, which integrates the advantages of the overpressured layer chromatography (OPLC) and bioautography. It exploits the interaction possibilities between the microbes andthe dye substance as well as other small and big co-factor molecules in the sorbent bed aver separation. OPLC separation resulted in the increase of the sensitivity and the compactness of the spots in comparison to the conventional TLC-HPTLC separations. OPLC and bioautography as a detection system facilitate studying different biological interactions in the field of antibiosis, especially model reactions of endogenous formaldehyde (HCHO) and other small endogenous molecules (e.g. H 2 O 2 ), as well as trans-resveratrol.