Developing biodegradable scaffolds for tissue engineering of the urethra

Abstract

This study adds to our understanding of how sterilization and cell seeding affect the physical properties of scaffolds. Both PAA and γ-irradiation appear to be suitable methods for sterilizing PLGA scaffolds, although both reduce the tensile properties of the scaffolds. Cells grow well on the sterilized scaffolds, and with our current protocol produce constructs which have ≈ 30% of the mechanical strength and elasticity of the native buccal mucosa. We conclude that sterilized PLGA 85:15 is a promising material for producing tissue-engineered buccal mucosa.