Identification of Antheridic Acid as an Antheridiogen in <italic>Anemia rotundifolia</italic> and <italic>Anemia flexuosa</italic>

Abstract

Antheridic acid was identified by retention time and full mass spectra from GCMS analysis as an antheridiogen in Anemia rotundifolia and A. flexuosa. In the dark spore germination assay, antheridic acid was active down to 10−10 and 5 × 10−12g.ml−1 in A. rotundifolia and A.flexuosa, respectively. In the antheridium formation assay, antheridic acid was active down to 10−10 g.ml−1 in both A. rotundifolia and A.flexuosa