Detection of beak and feather disease virus DNA in embryonated eggs of psittacine birds

Abstract

Psittacine beak and feather disease (PBFD) is a common viral disease of psittacine birds. The causative agent, beak and feather disease virus (BFDV) is a small circular single-stranded DNA virus belonging to the genus Circovirus. We report the detection of viral DNA predominantly in the heart, intestine and liver, but also in the testes and digestive organs. Additionally, BFDV transmission was investigated. We discuss the possibility that BFDV is transmitted horizontally. In our experiments, embryonated and non-embryonated budgerigar eggs were analysed for the presence of BFDV DNA. In 35.3% of non-embryonated and 20% of embryonated eggs viral DNA was detected, suggesting that BFDV can be transmitted horizontally and vertically.