Remote Multi-colour Fluorescence Imaging of Selected Broad-leaf Plants

Abstract

An imaging fluorescence lidar system was used for remote multi-spectral meas urements on selected broad-leaf plants cultivated in th e Botanical Garden of the University of Karl sruhe.The tech nique allows to characteri se vegetati on in terms of chlorophyll content via sensing the ch lorophyll fluorescence ratio F690/F735 and also by the blue to reel tluoresceiice ratio F450/F690 in ima ges of one or severa l leaves.Remotely evaluated data were compared with in vivo chlorophyll fluorescence emission spectra, meas ured in the near di stance up to 20 em, for the same leaf samples.In additi on, the leaves were physiologically characteri zed via determination of the variable chlorophyll fluorescence ratio, the Rfcl690-values.Leaves of different plants with different chlorophyll content were tested.We also studied the effect of a block of photosynthesis by the herbicide diuron on the flu orescence ratios F690/F735 and F450/F690.The results obtained indicate that fluorescence imaging may prove to be a valu able tool in the remote assessment of the vegetation status in relation to environmental stress and herbi cide treatment.