Applicability of non‐linear versus linear fractional abundance calibration plots for the quantitative determination of triacylglycerol regioisomers by tandem mass spectrometry

Abstract

The fractional abundance calibration plots used in regioisomeric analysis of triacylglycerols are intrinsically non-linear, but may appear linear if the scatter in data points obscures the curvature, if the curvature is slight, or if the response factors for the two isomers in the regioisomer pair are similar. Therefore, linearity should not be assumed for these types of measurements until confirmed experimentally.