Isolation and genetic characterization of Escherichia coli mutants defective in propionate metabolism

Abstract

Escherichia coli mutants defective in propionate metabolism (Prp-) were isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Prp- mutants demonstrate a phenotypic inability to grow on odd-chain-length fatty acids. The new genetic locus for the Prp- phenotype maps at approximately 98 min on the E. coli chromosome.