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The Effect of Tannic Acid on the Preservation of Tissue Culture Cells for Scanning Electron Microscopy
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1981
Year
Tissue EngineeringMicroscopyCell CultureBiomedical EngineeringDermatologyTissue Culture CellsOrthopaedic SurgerySkin RegenerationScanning Electron MicroscopyTa TreatmentHealth SciencesTannic AcidSkin SubstituteCell BiologyIn Vitro TechniquesWound HealingTissue CultureMedicinePlastic Surgery
Treatment with tannic acid and OsO4 (TA treatment) of tissue culture cells after fixation with glutaraldehyde and OsO4 resulted in good protection of the cell features against shrinkage and thermal damage caused by subsequent procedures used for specimen preparation. A difference in the degree of shrinkage between TA-treated cells and untreated cells was found, the mean diameter of the TA-treated cells being 1.4 times as large as that of untreated cells.