Technical Note: A procedure for the preparation and quantitative analysis of samples for titanium dioxide1

TLDR

The study developed a rapid method for determining titanium dioxide concentrations in feed and fecal samples. Samples are digested in concentrated H₂SO₄, treated with 30 % H₂O₂, and absorbance is measured at 410 nm using TiO₂‑spiked blanks to produce a linear standard curve. The method completes in ~4.5 h and achieves TiO₂ recoveries of 96–99 % compared to 53–84 % with the dry‑ash procedure, proving it to be a rapid, accurate alternative.

Abstract

A procedure was developed for the rapid analysis of titanium dioxide (TiO2) concentrations in feed and fecal samples. Samples were digested in concentrated H2SO4 for 2 h, followed by addition of 30% H2O2, and absorbance was measured at 410 nm. Standards were prepared by spiking blanks with increasing amounts of TiO2, resulting in a linear standard curve. Complete analysis using this procedure can typically be accomplished within 4.5 h. This procedure was compared to a previously published dry-ash procedure for the analysis of TiO2 in bovine fecal samples. Three sources of OM devoid of TiO2 (a forage sample, a bovine fecal sample without Cr2O3, and a bovine fecal sample containing Cr2O3) were spiked with graded amounts (0, 2, 4, 6, 8, or 10 mg) of TiO2. With our procedure, TiO2 recoveries averaged 96.7, 97.5, and 98.5%, for the three OM sources, respectively, vs. 74.3, 83.8, and 53.1% for the same samples analyzed using the dry-ash method. These results suggest that our procedure is a rapid and accurate alternative to dry-ash procedures for the determination of TiO2.