Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues.

TLDR

Bone marrow grafts under the renal capsule generate bone and marrow in syngeneic and semisyngeneic transplants, whereas allogeneic grafts produce only bone that is later resorbed. The study aims to discuss how preosteoblasts and hematopoietic stem cells interact to enable inducible bone formation. The authors used chromosome marker T6 and reverse transplantation to determine donor versus recipient origins of osteogenic and hematopoietic cells in semisyngeneic heterotopic bone marrow transplants. After 14 months, the bone marrow in semisyngeneic transplants is composed of recipient cells, while osteogenic precursors and bone tissue remain donor-derived.

Abstract

In semisyngeneic heterotopic bone marrow transplants the donor or recipient origin of cells of osteogenic and hematopoietic tissues was identified by chromosome markers (T6) and by reverse transplantation into the initial donor line. In syngeneic and semisyngeneic grafts of bone marrow under the renal capsule bone and bone marrow are formed. In allogeneic grafts only bone is formed; this bone is subsequently resorbed. In 14-month semisyngeneic transplants the bone marrow consists of recipient cells. This is true for both the proliferating pool and the stem cells of hematopoietic tissue. At the same time, osteogenic precursor cells and bone tissue in these transplants are of donor origin. A discussion is presented of the interrelationship between determinated osteogenic precursor cells (preosteoblasts) and hematopoietic stem cells (or their descendants) in which osteogenesis is inducible.