Microbial Drug Resistance · 2014 · 21 citations · 10 references
Life SciencesInfectious DiseasesMicrobiologyAntimicrobial ResistanceAntimicrobial Resistance EpidemiologyMedical MicrobiologyAntibiotic ResistanceAntibiotic Resistance GeneticsBacterial EpidemiologyBeta-lactam ResistanceResistance GenesVeterinary Antimicrobial ResistanceCanine Infectious DiseasesEscherichia ColiEscherichia Coli ResistanceExtended-spectrum Beta-lactamasesPrevalence VariabilityPulsed-field Gel ElectrophoresisQuinolone Resistance GenesQuinolone Resistance Plasmids
Antimicrobial resistance (AMR) represents a serious problem globally, but it is especially pronounced in the tropics, where pressure of infectious diseases is high. We examined resistance in Escherichia coli colonizing gastrointestinal tracts of 17 dogs which have never received antimicrobial treatment, living in central rural Angola. Emphasis was placed on extended-spectrum beta-lactamases (ESBL) and plasmid-mediated quinolone resistance (PMQR). Resistance-carrying plasmids were characterized in size, group of incompatibility and ability to conjugate. Isolates were compared by their pulsed-field gel electrophoresis (PFGE) profiles. Detailed description of 19 E. coli isolates with either ESBL or PMQR genes carried on multiresistant plasmids of different groups of incompatibility indicates that dogs, despite never being treated by antibiotics, are important reservoirs and transmitters of AMR in the study area.
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Identification of plasmids by PCR-based replicon typing
Alessandra Carattoli, Alessia Bertini, Laura Villa et al. · Journal of Microbiological Methods · 2005 · 2.3K citations
Sex and virulence in <i>Escherichia coli</i>: an evolutionary perspective
Thierry Wirth, Daniel Falush, Ruiting Lan et al. · Molecular Microbiology · 2006 · 2K citations · Full text