PubMed · 1993 · 18 citations · 0 references
Life SciencesGeneticsMolecular BiologyMolecular DiagnosticsNucleic Acid BiochemistryNucleic Acid ChemistryNucleic Acid ExtractionNucleic Acid PurificationPolymerase Chain ReactionAmplificationBlood Nucleic Acid ExtractionDna ExtractionNucleic Acid Sample PreparationPolymerase Chain Reaction (Pcr) AmplificationSilica-based Dna ExtractionSilica-based ExtractionSilica-based Purification
We describe a rapid, simple and inexpensive method for the isolation of DNA from blood clots suited for use in PCR. Our method is based on the lysing and nuclease-inactivating properties of guanidine thiocyanate together with the nucleic acid-binding properties of silica particles. Isolated DNA can be used for in vitro amplification as shown for a retinoblastoma gene PCR system.