Molecules and Cells · 2005 · 44 citations · 13 references
AgricultureGeneticsPlant SciencesBreeding For Disease ResistanceGermplasm EvaluationPlant GeneticsIntrogression Of Resistance TraitsResistance Gene DiscoveryResistance ManagementResistant Crop VarietiesBulk Segregant AnalysisBulked Segregant AnalysisIntrogression Of Resistance GenesMarker-assisted Selection For ResistanceMolecular Markers For ResistancePlant Resistance BreedingPlant Resistance GenesResistance Trait Evaluation
This study was carried out to identify a high-resolution marker for a gene conferring resistance to brown planthopper (BPH) biotype 1, using japonica type resistant lines. Bulked segregant analyses were conducted using 520 RAPD primers to identify RAPD fragments linked to the BPH resistance gene. Eleven RAPDs were shown to be polymorphic amplicons between resistant and susceptible progeny. One of these primers, OPE 18, which amplified a 923 bp band tightly linked to resistance, was converted into a sequence-tagged-site (STS) marker. The STS marker, BpE18-3, was easily detectable as a dominant band with tight linkage (3.9cM) to Bph1. It promises to be useful as a marker for assisted selection of resistant progeny in backcross breeding programs to introgress the resistance gene into elite japonica cultivars.
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