Human apolipoprotein E. The complete amino acid sequence.

Abstract

The amino acid sequence of human apolipoprotein E (apo-E) has been determined.Apo-E2, one of the major isoforms of apolipoprotein E, is a polypeptide of 299 amino acids having a calculated molecular weight of 34,145.The isoform apo-E3 differs from apo-E2 by a single amino acid substitution of arginine for cysteine at residue 158 (italicized).The sequqnce of apo-E2 is:HzN-Lys-Val-Glu-Gln-Ala-Val-Glu-Thr-Glu-Pro-Glu-Pro-Glu-Leu-Arg-Gln-Gln-Thr-Glu-Trp-Gln-Ser-Gly-Gln-Arg-Trp-Glu-Leu-Ala-Leu-Gly-Arg-Phe-Trp-Asp-Tyr-Leu-Arg-Trp-Val-Gln-%-Leu-Ser-Glu-Gln-Val-Gln-Glu-Glu-Leu-Leu-Ser-Ser-Gln-Val-Thr-Gln-Glu-Leu-Arg-Ala-Leu-Met-Asp-Glu-Thr-Met-Lys-Glu-Leu-Lys-Ala-Tyr-Lys-Ser-Glu-Leu-Glu-Glu-Gln-Leu-Thr-Pro-Val-Ala-Glu-Glu-Thr-Arg-Ala-Arg-Leu-Ser-Lys-Glu-Leu-Gln-Ala-Ala-Gln-Ala-Arg-Leu-Gly-Ala-Asp-Met-Glu-Asp-Val-Cys-Gly-Arg-Leu-Val-Gln-Tyr-Arg-Gly-Glu-Val-Gln-Ala-Met-Leu-Gly-Gln-Ser-Thr-Glu-Glu-Leu-Arg-Val-Arg-Leu-Ala-Ser-His-Leu-Arg-Lys-Leu-Arg-Lys-Arg-Leu-Leu-Arg-Asp-Ala-Asp-Asp-Leu-Gln-Lys-Cys-Leu-Ala-Val-Tyr-Gln-Ala-Gly-Ala-Arg-Glu-Gly-Ala-Glu-Arg-Gly-Leu-Ser-Ala-Ile-Arg-Glu-Arg-Leu-Gly-Pro-Leu-Val-Glu-Gln-Gly-Arg-Val-Arg-Ala-Ala-%-Val-Gly-Ser-Leu-Ala-Gly-Gln-Pro-Leu-Gln-Glu-Arg-Ala-Gln-Ala-Trp-Gly-Glu-Arg-Leu-Arg-Ala-Arg