RFLP relationships of A-genome species in the genus Oryza

Abstract

RFLPs of 192 accessiens ef A-genome rice species were aRalyzed ORe plant vvas used to represent each accession assayed RFLPs were detected for the combinations of Dra 1-digested total DNA and tweRty-one sirgle-copy genomic cones A dendrogram was constructed using ePGAryiethd frrl a geRetic distaxceaxasscafhe A-geRmece speces based on RFLP analysis matched well with the conventional classcation The African annual species O gtaberrimct and O barthii were not clearly differentiated by RFLP loci while the Assecies a 3v O)ogen and Ozivara f)ed a cex wheee ofe species can easily be distinguished from each other An Asian sub-group that is clearly differentiated from other Asian A-genome germplasm was identified This consists of some O gon accessions that dd nt cster withe cutivaed rce buomed 1sely k groups Some accessions of O(were closely aned to(ca rice cultivars whe the others mainly from China were closely aligned with Japonica cultivars Most of O accessions from Latin America foed a group which was more closely reled to O glaberriza aRd O barShii thak other species Two accessieRs gf O ggecmaepatecga however were aligned with lndica cultivars of Asia lt is possible that the A-genome wild rices in Latin America are of two types O rufipogoz from the Sepik river of Papua New Guinea was eaterta1whhad RFLPgesRy